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Simply Cape Cod's best location!

Simply Cape Cod's best location!

Simply Cape Cod's best location!

A conference about JavaScript

Tickets available • EUR 159 (incl. VAT)

Creator of Vue.js, Hackensack, NJ

Deque Systems, Bellingham, WA

Microsoft, London, UK

RxJS core team, Creator of Cycle.js, Helsinki, FI

Netlify, St. Albans, UK

Geildanke.com, Berlin, DE

Thoughtworks, Sydney, Australia

If the form below is not visible or not working for you, please use this link to get your conference ticket.

All prices include VAT. Buying via Tito also gets you an invoice. Tickets are not refundable, but re-assignable.

Script is a 100% independent one-day, curated and handcrafted JavaScript event for the community, happening in Linz, Austria. Join us on January 19, 2018 for a day (and night) of fun, inspiration and education.

ScriptConf in Linz, Austria – if you want all the good with none of the drama. https://www.christianheilma nn.com/2017/02/15/scriptconf-in-linz-austria-if-you-want-all-the-good-with-none-of-the-drama/ - thank you womens nike khaki air max thea trainers club

Chris "codepo8" Heilmann, Microsoft

If you want to see what awaits you at Script'18, check out our nike air max thea woman red cape
. It contains all the details on our event.

Doors and registration

Opening

Evan You will talk on

The Journey to Full Time Open Source

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will talk on

Radically Accessible Internet Applications 💯

Coffee break powered by epunkt

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will talk on

Hop on the serverless adventure with NodeJS

Michaela Lehr will talk on

Creating Augmented Reality Apps with Web Technology

Andre Staltz will talk on

Cycle.js on pen and paper

Dinner powered by karriere.at

Phil Hawksworth will talk on

Next wave infrastructure - do far more with much less

Charlie Gerard will talk on

Look mum, no hands!

Closing + Party 🎉 powered by Dynatrace

Script'17 takes place at Central Linz , Landstraße 36, 4020 Linz. Originally one of the first cinemas in Linz, it has become one of the top locations for concerts and events in the steel city.

The Central lives up to its name and is in the center of Linz, directly next to the Mozartstraße tram station. See how to get to the venue and to Linz in general air jordan 13 designer jewelry
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Cell Ranger 1.2 and later support libraries generated by the Chromium Single Cell 3′ v1 and v2 reagent kits, whereas Cell Ranger 1.1 and earlier do not support v2 libraries. Support for 5′ reagents is new in Cell Ranger 2.1.

The Cell Ranger workflow starts with demultiplexing the raw base call (BCL) files for each flowcell directory. 10x recommends using cellranger mkfastq as described in Generating FASTQs . However, it is possible to get compatible FASTQ files other ways, such as by starting with a public dataset which provides them, or using foot locker nike free 40 flyknit 2015 movies
. In that case, you would skip mkfastq and begin with cellranger count . Please see the Specifying Input FASTQs page for specific guidelines on which arguments to use for your scenario.

The subsequent steps vary depending on how many samples, libraries, and flowcells you have. We will describe them in order of increasing complexity:

This is the most basic case. You have a single biological sample, which was prepared into a single library, and then sequenced on a single flowcell. Assuming the FASTQs have been generated with cellranger mkfastq , you just need to run cellranger count as described in cheap roshes for women
.

If you have a library which was sequenced across multiple flowcells (e.g. to increase sequencing saturation), you can pool the reads from both sequencing runs. Follow the steps in air jordan 6 black infrared nike
to combine them in a single cellranger count run.

This comprises two scenarios.

If you prepared multiple libraries from the same sample (technical replicates, for example), then each one should be run through a separate instance of cellranger count . Once those are completed, you can perform a combined analysis using cellranger aggr , as described in Multi-Library Aggregation . (This is illustrated in the figure).

If you prepared multiple libraries from the same sample and want to pool them and analyze the combined data as a single sample, then you will need to use the nike roshe run original vs fake vans
to treat the multiple libraries as coming from a single sample. (This is not illustrated in the figure).

we love this music

April 04, 2018

Portland, OR 53° F

Each week, All Classical Portland presentsThursdays @ Three, live broadcast concerts showcasing local musicians, arts partners, and visiting artists. Christa Wessel hosts the lively format of music-making and conversation for members of an intimate studio audience. That’s what we call Building Cultural Community!

Visit the nike air force white pack foamposites
to listen to recent Thursdays @ Three performances!

Thursdays @ Three concerts are broadcast live and in real time from our studios in the Hampton Opera Center, and programs last about an hour. If you would like to join our studio audience, please select an event below. These are small group audience experiences that fill up fast. Seating is available on first come, first served basis.

If you would like to join our studio audience, please select an event below.

We kindly request that you limit your attendance to one Thursdays @ Three performance per month .

We kindly request that you limit your attendance to one Thursdays @ Three performance per month

April 5th, 2018 Jasper Wood, David Riley, Trinity Music

April 5th, 2018 Jasper Wood, David Riley, Trinity Music

Canadian violinist Jasper Wood and pianist David Riley join forces on Thursdays @ Three. Gramaphone writes of the duo:“Encores there must be wherever Wood and Riley play: theirs is a beautiful collaboration. Wood is always a joy to hear.” Additionally, Trinity Music offers a preview of their upcoming collaboration with Portland Baroque Orchestra on Bach’s B minor mass.

Sorry, seating is no longer available for this performance.

April 12th, 2018 Classical Up Close

April 12th, 2018 Classical Up Close

Classical Up Close is a collective effort by members of the Oregon Symphony and friends to bring great classical music to the whole community. These artists want as many people as possible to have access to this beautiful music, so they go into neighborhoods and workplaces all around the Metro area, and every event is absolutely free!​ Hear members of the Oregon Symphony preview their 2018 Classical Up Close Festival!

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ABN: 15 211 513 464
CRICOS Number: 00026A
ABN: 15 211 513 464
CRICOS Number: 00026A